Ozone and Dentistry: Applications in Dental Treatments

Did you know that Ozone Therapy can be applied in dental treatment? Ozone in dentistry is a remarkable solution to reduce the use of chemicals and to give you healthier teeth. A dental treatment with ozone has several benefits, including disinfection and pain-relief properties.

So how can ozone be used in dentistry? Scroll down and you’ll learn!

Ozone is a powerful oxidant that effectively affects bacteria, fungi, viruses and parasites at a much lower concentration than chlorine without any of its toxic side effects because at the end of its performances it returns to the form of oxygen.

A high concentration of ozone kills bacteria very quickly and is a thousand times more powerful than other bacterial killing agents. An ozone molecule is equal to 3,000 to 10,000 molecules of chlorine that acts 3,000 times faster killing pathogens.

There are several known actions of ozone in the human body, such as immunostimulants and analgesics, antihypoxic and detoxifying, antimicrobial, bioenergetic and biosynthetic (activation of metabolism of carbohydrates, proteins, lipids).

Dentistry has been experiencing a period of dynamic changes and growth. The dental ozone therapy treatment is becoming a common practice in some countries. Also, this therapy has been more accepted and beneficial than present conventional therapeutic modalities because it is a minimally invasive treatment compare to the traditional treatments.

Ozone therapy has a wide range of applications in almost every field of dentistry. Its unique properties include immunostimulant, analgesic, detoxicating, antimicrobial, bioenergetic, and biosynthetic actions.

Natural, safe, and effective, the ozone dental therapy achieves excellent results with no risk of allergic reactions or contraindications. Furthermore, ozone is applied at the time of treatment, eliminating bacteria immediately and reducing the recommended medication time.

Ozone therapy is a promising treatment modality for various dental problems in proximal future. Use of ozone in dentistry:

  • Periodontology – Gingivitis, Periodontits, Periimplantitis, Surgical cuts, Prophylaxis;

  • Dental and oral pathology – Caries, Enamel cracks, Root canal treatment, Tooth whitening, Dentinal hypersensitivity, Abscess, Granuloma, Fistulae, Apthae, Herpes infection, Stomatitis – Candidiasis;

  • Surgery – Implantation, Re-plantation, Extraction, Wound Healing, Coaguloapathy – prolonged bleeding;

  • Prosthodontics and restorative dentistry – Stumps and crown disinfection, Cavity disinfection;

  • Orthodontics and orthopedics – TMJ dysfunctions, Trismus, Relaxation, Myoarthtopathy;

  • Diagnostics – Vitality test.

It is also important to highlight that Ozone therapy has contra-indications. Always be in touch with your dentist or doctor, before introducing the therapy in your healthcare.

Studies have shown that 99 percent of all the bacteria causing tooth decay have been eliminated after 10 seconds of ozone exposure and even 99.9 percent bacteria after 20 seconds exposure.

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Dental treatment modalities of Ozone Therapy


The impact of ozone has been recognized in several studies on various types of caries (pits and fissures, non-cavities and primary root caries). Interesting data shows that ozone does not affect the sealing capacity of the glue system. Ozone therapy has also been used to prevent dental caries in the first permanent molar fissures in children.

Ozone in endodontics focus on its antimicrobial activity. Since polymicrobial infections complicate cases of apical periodontitis, ozone, due to its oxidative power, is characterized as an efficient antimicrobial and its indication for use in endodontic therapy seems quite appropriate. Its antimicrobial action has been demonstrated against bacterial strains such as MycobacteriaStaphylococcusStreptococcusPseudomonasEnterococcusE. coliS. aureusE. faecalis and C. albicans using in vitro research models.

  • Hyper-Sensitive Teeth – Enamel and Dentine loss occurring due to multiple factors like attrition, abrasion, erosion, trauma from occlusion may cause hyper sensitivity and diagnosis of etiology is vital. After elimination of cause an ozone application is found to instantly reduce pain in these sensitive teeth. Ozone removes the smear layer, opening the dentinal tubules, widening its diameter and then the calcium and fluoride ions flow into the tubules in an easy, deep and effective way to cover the dentinal tubules, preventing the exchange of fluids through these tubules. In this way, ozone quickly eliminates the problem of root sensitivity and its effect lasts longer than conventional methods.

  • Proximal Caries Lesions and root caries – The application of ozone significantly reduces the presence of S. mutans, however, longer applications are also very efficient in the elimination of cariogenic species such as S. mutansL.casei and A.naeslundii in carious root lesions. However, if there are carious lesions that can be detected on the X-ray, the exposure to ozone has to be increased and has to be followed by a restoration.

  • Bleaching – Ozone can be used for tooth bleaching with very satisfactory results. After root canal treatment, crown discoloration is a major aesthetic problem. Ozone was been successfully used for lightening the yellowish tinge. The bleaching effect with ozone is seen when the bleaching agent is placed in the access cavity & crown is exposed to ozone.

  • Vital Root Canal Therapy – After effective biomechanical preparation, irrigation with Ozonated water significantly reduces the bacterial population in the root canal.


Gengival and Periodontal diseases represent a major concern both in dentistry and medicine. The dental biofilm makes the use of antibiotics in the direction of putative periodontal pathogens, requiring higher concentrations of antibiotic to achieve positive results. The application of ozone in periodontics has shown promising results as far as the aqueous ozone gas are used as substitutes mechanical debridement.

Ozonized water (4mg / l) inhibits dental plaque formation and reduces the number of subgingival pathogens, both gram-positive and gram-negative organisms. Moreover, ozonated water has a strong bactericidal activity against bacteria in the biofilm plates and inhibits plaque accumulation.

The application of ozone on gingival and periodontal chronic diseases have revealed improvements of their condition, as well as patients with periodontal abscess without exudation.

Ozonated water can be used:

  • as a powerful disinfectant;

  • in its ability to control bleeding;

  • in its ability to cleanse wounds in bones and soft tissues;

  • to improve healing, by increasing the local supply of oxygen to the wound area;

  • to increase temperature in the area of the wound, and this increases the metabolic processes related to wound healing.


Soft tissue lesions like herpes, aphthae, removable denture ulcers, cuts, cheilitis, candidiasis, cysts and traumatic wounds can be treated with either Ozonated water or oils. The disinfectant and healing properties help in the healing of these lesions.


Ozone is known to encourage wound healing as well as control opportunistic infection. The influence of ozonated water on the healing process of epithelial wounds in the oral cavity was observed by A. Filippi (in “PCNA expression of cementoblasts and fibroblasts on the root surface after extraoral rinsing for decontamination. Dent Traumatol 2002”).

After a tooth is extracted or any surgical procedure the area is irrigated and insufflated which promotes faster healing without complications. It has been found that daily applied ozonated water can accelerate the healing rate in the buccal mucosa. This effect can be observed on the first two postoperative days without the need for systemic medication. Ozonized oil may be effective in treating alveolitis. After tooth extraction and in case of post-extraction complications ozone is very useful both in disinfection and elimination of bacteria and in the acceleration of the healing process.

  • Chronic or recurrent infections in the oral cavity;

  • Eliminates bacterial pathogens (biofilm purging);

  • Reduces infections of denture prosthetics of fungus, yeast, bacteria and virus;

  • Periodontal pocket disinfection and osseous disinfection;

  • Dental caries prevention and remineralization;

  • Endodontic treatments;

  • Tooth extraction;

  • Tooth sensitivity;

  • Gum recession (exposed root surfaces);

  • Pain control;

  • Infection control;

  • Plaque control;

  • Pre-washing of surgical sites and disinfects post-extraction;

  • Accelerated healing;

  • Tissue regeneration;

  • Controlling bad breath (halitosis);

  • Remineralization of tooth surface;

  • Bleaching teeth;

  • and much more.


Because of its high oxidation potential, it is effective against various microorganisms like bacteria, viruses, fungi, and protozoa which are necessary for plaque maintenance.

Ozone has been established as a potent denture cleansing agent due to its antiplaque properties. The bacterial plaque that accumulates in the dentures is composed of several oral microorganisms, mainly C. albicans.

Denture stomatitis is routinely encountered in clinical practice which is a manifestation of plaque accumulation on the surface of the denture and hence effective denture plaque control should be initiated to prevent such outcomes. One successful method to do so is the use of ozone as denture cleaner. The control of the denture plate is essential for the prevention of prosthetic stomatitis.

The application of ozonated water in combination with ultrasonography in C. albicans has been shown to be a viable solution in reducing the number of C. albicans.


There are several benefits to use ozone therapy because ozone is a powerful and natural agent that kills bacteria, fungus, viruses and parasites with antiseptic properties that makes it a wonderful tool to disinfect.

In dentistry, ozone is a fantastic tool to disinfect surgical and extraction sites, implant perpetration sites and areas of infections, leaving no unpleasant side effects.

Using ozone, improves healing, decreases inflammation, pain and swelling in the treated area, preserving the integrity of natural teeth as much as possible.

There are several ways to apply ozone during dental treatments without taking away their properties:

  • As a hygienizer making regular mouthwash during treatment;

  • As infusion in the infected maxillary bone (cavitation);

  • As an infusion in the temporomandibular joint for the treatment of pain and inflammation;

  • As an irrigant with high concentrations during surgical procedures;

  • As an irrigant with high concentrations during the new root canal therapy to disinfect the involved tooth;

  • Use of ozonated olive oil for periodontal disease (adjunctive therapy);

  • and much more.

Thus, the dental ozone therapy decreases the treatment time, it eliminates the bacteria and parasites more precisely and, moreover, it is completely painless.