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Ozone, a fantastic solution for Surface Sanitation

If you are looking for surface sanitation solutions, Ozone is your answer! One of the several properties of Ozone is the strong oxidizing agent. Because of that, Ozone is the best option when it comes to surface sanitation, whether it is in food safety sanitation, ozone applied to water or pool sanitation, or in countertops in our daily lives or food processing industry, Ozone comes as a great sanitation solution.

If you want to learn more about how great ozone is as a disinfectant, keep scrolling and read it all below!

Adopting ozone in cleaning and disinfection process can bring many advantages over commonly disinfectants. Ozone can be one of the most effective sterilizing tools.

Ozone is the most powerful oxidative agent that occurs naturally. It is able to destroy germs, virus, bacteria and microbes that may cause surface or air contaminations. Furthermore, ozone leaves no chemical residue typical of alternative detergent or synthetic cleaners because it breaks down quickly into oxygen. However, it must to be handled properly.

It is a strong disinfectant and it is increasingly used for infection protection and multiple sterilization applications: to sanitize laboratory tools, especially ones involved in sensitive experiments; It is effective to sterilize pipettes, gloves, plates, small equipment and even personal items such as keys and glasses. Also, surfaces, such as floors, walls, counter tops, conveyer belts, fruit bins, crushers, pipes can be sanitized with direct application of ozone and water.

How does it work?

Ozone is able to kill 99.7% of 650 different kinds of pathogenic organisms (bacteria and fungi) and it is very safe and efficient. It can penetrate cavities or crevices that normally are hard to clean and no rinse is needed after ozone application, as ozone will leave no residual on the surface. This may lower cleaning time and water usage costs.

Because ozone requires no storage or special handling and does not need to be mixed with other products, it may be viewed as advantageous over other chemical sanitizers. Also, ozone is used for surface sanitation in many food processing applications, because it is a powerful oxidant that kills all bacteria on contact.

In food processing it is essential to keep food products pathogen free and reduce the potential for cross-contamination of deadly pathogens. Therefore, the surface sanitation is very important. One of the major concerns for cross-contamination on food processing equipment is biofilm build-up.

Biofilms are layers of microorganisms that grow up tightly in the surfaces. What happens is the new layer of microbes can provide nutrients and protection against sanitizers, and it is a concern because over time it makes sanitation more difficult. These resistant biofilms are most common in cracks, crevices, conveyors, knives, slicers, and portioners and corners of food processing equipment that are only sanitized periodically.

Because of the strong disinfection power of ozone, the biofilm can be removed by ozonating over an extended time period, allowing ozone to break down cell walls and organic material and prevent future growth.

Ozone Surface Disinfection

ApplicationMicro-organismTreatmentResultsAuthor, Year
CIP systemStaphylococcus aureus, Pseudomonas aeruginosa and Candida albicansOzonated water99% microbial count reductionLagrange, Reiprich, & Hoffmann, 2004
Dairy biofilms on stainless steel surfacePseudomonas fluorescens and Alcaligenes faecalisOzonated water, 0.5 ppm for 10 min5.6 and 4.4 log reduction, respectivelyGreene, Few, Joao, & Serafini, 1993
Equipment, walls, floors, drains, tables and conveyors, previously well-cleanedTrichophyton mentagrophytes, Salmonella choleraesuis, Staphylococcus aureus, Pseudomonas aeruginosa, Campylobacter jejuni, Listeria monocytogenes, Aspergillus flavus, Brettanomyces bruxellensis, Escherichia coliOzonated water, 3.0 - 3.5 ppmLog reduction ranging from 6 to 4Boisrobert, 2002
‘High-traffic’ and ‘low-traffic’ floor areasUnspecifiedOzonated water, 2 ppm at 10 gpm for 1 minMicrobial plate count reductions 67.0 - 95.6%Hampson, 2000
Mixing kettle, table top and shroud (all stainless steel)UnspecifiedOzonated water, 2 ppm at 10 gpm for 1 minMicrobial plate count reduction ranging from 63.1 to 99.9% (depending on surface)Hampson, 2000
Plastic shipping containerUnspecifiedOzonated water, 2 ppm at 10 gpm for 1 minMicrobial bioluminescence assay reduction 68.8 - 97.4%Hampson, 2000
Stainless steel surfacesEscherichia coli, Serratia liquefaciens, Staphylococcus aureus, Listeria innocua and Rhodotorula rubra2 ppm ozone gas at atmospheric pressure, 22 ˚C and 77% HR for 4 hReduction ranging from 7.56 to 2.41 log valuesMoore, Griffith, & Peters, 2000
Stainless steel surfacesMicroccocus luteus2 ppm ozone gas in bioaerosol chamber at 20˚C and 50% HR for 1 h2 - 3 log reductionBailey, Young, Fielding, & Griffiths, 2001
Stainless steel surfaces in the presence of UHT milkEscherichia coli, Serratia liquefaciens, Staphylococcus aureus, Listeria innocua and Rhodotorula rubra2 ppm ozone gas at atmospheric pressure, 22 ˚C and 77% HR for 4 hReduction ranging from 5.64 to 1.65 log valuesMoore et al., 2000
SurfacesUnknown2 ppm ozone gas, 2 h exposure2 log reductionTaylor & Chana, 2000

Source: A. Pascual, I. Llorca, & A. Canut. (2007). Use of ozone in food industries for reducing the environmental impact of cleaning and disinfection activities. Trends in Food Science & Technology 18, pp. S29 – S35.

Benefits

    • Cleaning and sanitizing agent;

    • Eliminates or reduces microscopic organisms;

    • Sterilizes any surfaces and air spaces in multiple areas;

    • Sterilizes any tool and equipment (labs, industrial, food processing);

    • Safe and efficient process use;

    • Leaves no harmful by-products;

    • No toxic emissions;

    • No residue to aerate;

    • Eliminates need for harmful liquids, chemicals, UV rays, or damaging heat;

    • No rinsing required;

    • Produced onsite;

    • Low operating temperature;

    • No danger of an accidental burn;

    • Highly cost effective;

    • Save water and energy;

    • Save wastewater treatment costs and discharge taxes;

    • Ozone decomposes rapidly and naturally, and therefore, it leaves no harmful residual that would need to be removed.

Scientific Articles

Published: 2023

EMMANUEL I. EPELLE, ANDREW MACFARLANE, MICHAEL CUSACK, ANTHONY BURNS, JUDE A. OKOLIE, WILLIAM MACKAY, MOSTAFA RATEB, MOHAMMED YASEEN | February 15th | Ozone application in different industries: A review of recent developments

Published: 2021

SOUMYA N. MANJUNATH, M. SAKAR, MANMOHAN KATAPADI, R. GEETHA ALAKRISHNA  | February | Recent case studies on the use of ozone to combat coronavirus: Problems and perspectives

INES ZUCKER, YAAL LESTER, JOEL ALTER, MICHAL WERBNER, YINON YECHESKEL, MEITAL GAL-TANAMY, MOSHE DESSAU  | January 13th | Pseudoviruses for the assessment of coronavirus disinfection by ozone

IOA (The International Ozone Association) | 2021 | Air Treatment

MAICON H. CAETANO, JOÃO P. Z. SIQUEIRA, DENISE DE ANDRADE, Á. F. L. DE SOUSA, MARCELO A. RIGOTTI, MAIARA O. DINIZ, WILLIAM A. DE ALMEIDA, MARGARETE T. G. DE ALMEIDA | 2021 | Antimicrobial action of ozone gas on surfaces and in the air

Published: 2020

ELENA GRIGNANI, ANTONELLA MANSI, RENATO CABELLA, PAOLA CASTELLANO, ANGELO TIRABASSO, RENATA SISTO, MARIANGELA SPAGNOLI, GIOVANNI FABRIZI, FRANCESCO FRIGERIO, GIOVANNA TRANFO| December 24th | Safe and Effective Use of Ozone as Air and Surface Disinfectant in the Conjuncture of Covid-19

GIUSEPPINA MOCCIA, FRANCESCO DE CARO, CONCETTA PIRONTI, GIOVANNI BOCCIA, MARIO CAPUNZO, ANNA BORRELLI, ORIANA MOTTA | November | Development and Improvement of an Effective Method for Air and Surfaces Disinfection with Ozone Gas as a Decontaminating Agent

FELIPE AGOSTINI, MELISSA FACCINI, FRANCISCO FITARELLI & OTHERS | October 1st | In Vitro Comparison of Antibacterial Effect of Ozonated Water and Ozonated Gas

GOVINDARAJ DEV KUMAR, ABHINAV MISHRA, LAUREL DUNN, ANNA TOWNSEND, IKECHUKWU CHUKWUMA OGUADINMA, KELLY R. BRIGHT, CHARLES P. GERBA| June 23rd | Biocides and Novel Antimicrobial Agents for the Mitigation of Coronaviruses

Published: 2019

H. J. BREIDABLIK, D. E. LYSEBO, L. JOHANNESSEN, Å. SKARE, J. R. ANDERSEN, . T. KLEIVEN | August | Ozonized water as an alternative to alcohol-based hand disinfection

Published: 2017

M. MARTINELLI, F. GIOVANNANGELI, S. ROTUNNO, C. M. TROMBETTA, E. MONTOMOLI | March | Water and air ozone treatment as an alternative sanitizing technology

Published: 2012

BELCHOR FONTES, ANA MARIA C. HEIMBECKER, GLACUS DE SOUZA BRITO, SILVIA F. COSTA, INNEKE M. VAN DER HEIJDEN, ANNA S. LEVIN, SAMIR RASSLAN | December 18th | Effect of low-dose gaseous ozone on pathogenic bacteria

M. PRABAKARAN, SELVI S. TAMIL, S. MERINAL, A. PANNEESELVAN | 2012 | Effect of ozonation on pathogenic bacteria

Published: 2010

CHIH-SHAN LI, YU-CHUN WANG | June 4th | Surface Germicidal Effects of Ozone for Microorganisms

Published: 2009

JAMES B. HUDSON, MANJU SHARMA, SELVARANI VIMALANATHAN | May 27th | Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent

Published: 2008

MANJU SHARMA, JAMES B. HUDSON  | October | Ozone gas is an effective and practical antibacterial agent

C. A. GARCIA,  L. STANZIOLA, I. DE S. VIEIRA, J. H. F. DE F. NAVES, S. M. N. NEVES | July-December | The ozone gas in the decontamination of surgical environments

Published: 2007
Published: 2002

BENJAMAS THANOMSUB, VIPAVEE ANUPUNPISIT, SILCHAI CHANPHETCH & OTHERS | September | Effects of ozone treatment on cell growth and ultrastructural changes in bacteria

Published: 2000
Published: 1999
Published: 1987

J. M. VAUGHN, Y. S. CHEN, K. LINDBURG, D. MORALES | September | Inactivation of Human and Simian Rotaviruses by Ozone

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